Composite

Part:BBa_K5089006

Designed by: Camille Prefontaine   Group: iGEM24_UManitoba   (2024-09-29)


CLE-CsgA-LCI

This composite part consists of all components required for expressing and displaying CLE and LCI on the cell surface of E.coli BL21(DE3). The characterization of the enzymatic activity of CLE is available in the documentation for the base part BBa_K1149036. The characterization of LCI binding ability can be found in the documentation of the base part BBa_K3013000.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 226
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None